Ozone is a form of alternative medicine treatment that purports to increase the amount of oxygen through the introduction of ozone into the body. It is an allotropic form of oxygen, which is successfully used in the treatment of different diseases for more than hundred years. Ozone (O3) is an unstable gas and it quickly gives up nascent oxygen molecule to form oxygen gas. The use of ozone in dentistry represents an absolutely new solution of acute problems in the treatment of diseases of both therapeutic and surgical profile. Owing to the anti-inflammatory, immunomodulating effects of ozone and its positive influence on the microcirculation, the ozone therapy is now widely used in dental field. There are several potential actions of Ozone, which are applied in the clinical practice of dentistry and medicine, such as antimicrobial i.e. bactericidal, viricidal and fungicidal, anti-inflammatory, immunostimulating, antihypoxic and detoxicating, biosynthetic, (activation of the metabolism of carbohydrates, proteins lipids) bio energetics and hemostatic etc.Treating patients with ozone cuts off treatment time, eliminates bacterial count more precisely.