Microbial fuel cell moreover represents a genuine methodology for simultaneous waste treatment and bio-electricity generation. Microbial fuel cells (MFCs) are Instruments that can install bacterial metabolism process to produce an electrical current from a wide range organic substrates provided if they have capability to utilise it as substrate. Present study consists electricity generation from various bio-wastes such as Sewage Water, Paneer Whey and Rice Water which earlier considered as waste product, using a specially designed double chamber microbial fuel cell Chamber. The effect of Copper nanoparticle, anode and cathode surface area and various chemical treatments of electrodes on generated voltage and current by specially designed microbial fuel cell was investigated and recorded.